Table 2. LC-MS/MS Quantification of Citalopram and the Metabolite Desmethylcitalopram in Brain and Serum^a.

	brain (μM)			serum (μM)
	45 min	2 h	ANOVA	45 min	2 h	ANOVA
citalopram	5.52 ± 0.54	2.85 ± 0.38	*	0.76 ± 0.04	0.42 ± 0.05	*
desmethylcitalopram	0.38 ± 0.03	0.73 ± 0.12	**	1.03 ± 0.04	0.51 ± 0.10	*
desmethylcitalopram from citalopram	0.14 ± 0.01	0.37 ± 0.05	*	0.33 ± 0.01	0.46 ± 0.09	n.s.

^aAge-matched C57BL/6J mice were injected with either citalopram or desmethylcitalopram and sacrificed at 45 min or 2 h post-injection. The analytes were isolated from whole brain or serum and detected by LC-MS/MS (n ≥ 2 mice; values represent mean ± S.E.; one-way ANOVA with post-hoc Holm-Šídák multiple comparison test was used to compare 45 min to 2 h, *p < 0.05, **p < 0.01; n.s. not significant).