Table 2.

Oligonucleotides used in this study

Oligonucleotide	Sequence (5’ to 3’)a
0498LA-F (SalI)	CCCGTCGACGAAAGTGCGTCCAGCGTAT
0498LA-R (BamHI)	CCCGGATCCTTTGCTCTGCACGTTCTGC
0498RA-F (KpnI)	CCCGGTACCACTTCGCTCAAAATAATCCTAAT
0498RA-F (NheI)	CCCGCTAGCACCTAAGTCTAAACCTCCACAAC
Kan-F (BamHI)	CCCGGATCCGAACCATTTGAGGTGATAGG
Kan-R (KpnI)	CCCGGTACCGGTACTAAAACAATTCATCCAG
0498-F	GGAAGAACATCTGCTGGAA
0498-R	AGCTACGATAATTTAGTGCC
0497-F	TTTGTGGCTGAGAATGTTAA
0497-R	GTATTGGTGCTTGTCTACCTG
SpcR-F (KpnI)	CCCGGTACCAGAGTTGGTAGCTCTTGATCCG
SpecR-R (EcoRI)	CCCGAATTCTTTAAAAGTAAGCACCTGTTATTC
amyLA-F (SalI)	CCCGTCGACGAATAATGGGCAAATTCAGTGG
amyLA-R (BamHI)	CCCGGATCCAGAAGCGTCTTGTTCTTGACGA
amyRA-F (EcoRI)	CCCGAATTCATTTCACAAAGAAAATAGCA
amyRA-R (NheI)	CCCGCTAGCGGATGTGATTTACCAAAGTCT
C0498-F (BamHI)	CCCGGATCCGTATTCCAGACTGTTTGGAGCT
C0498-R (KpnI)	CCCGGTACCCTCTATCTAAATCAGGAGCAGGTT

^aRestriction enzyme recognition sites are underlined