Microbial community composition of selected original soil samples (0.5, 5, 12, and 120 kyr soil age) along the Franz Josef chronosequence based on pyrosequencing analysis. Relative abundances (%) of (A) archaeal groups, and (B) bacterial phyla and classes (Proteobacteria). (C) Principal component analysis (PCA) of archaeal community composition based on relative abundances of archaeal groups with soil age and soil depth as supplementary variables (gray arrows). 1.1c, Thaumarchaeota 1.1c; Bathy, Bathyarchaeota; FSCG, Forest Soil Crenarchaeotal Group; SAGMCG-1, South African Gold Mine Crenarchaeotal Group 1; Thaum_uncl, unclassified Thaumarchaeota, and Thpla, Thermoplasmatales. (D) PCA of bacterial community composition based on log-transformed relative abundances of bacterial genera (15 best-fitting as blue arrows) with soil age and soil depth as supplementary variables (gray arrows). Acithe, Acidothermus; Desulspo, Desulfosporosinus; GAL15, candidate division GAL15; Gem, Gemmata; Geobac, Geobacter; Leptspi, Leptospirillum; Naut, Nautilia. Uncultured genera belong to 7, Acidobacteria; 14, Chloroflexi; 33, 35, Nitrospirae; 41, Planctomycetes; 42, 43, Alphaproteobacteria; 48, Deltaproteobacteria. Symbol colors encode the different soil ages.