Table 1.

Strains and plasmids used to make the deletion mutations.

Strain, plasmid, or primer	Description	Source
S. aureus strains
SA2	MRSA carrying mutation on glpT and uhpT, fosfomycin MIC > 1024 μg/ml	Clinical strain 2 (Fu et al., 2016b)
SA94	MRSA carrying mutation on uhpT, fosfomycin MIC = 256 μg/ml	Clinical strain 94 (Fu et al., 2016b)
SA30	MRSA carrying mutation on glpT, fosfomycin MIC = 128 μg/ml	Clinical strain 30 (Fu et al., 2016b)
Newman	A fosfomycin-sensitive S. aureus strain, fosfomycin MIC = 0.5 μg/ml	Bae and Schneewind, 2005
RN4220	A non-a-hemolytic, non-restricting strain of S. aureus	Bae and Schneewind, 2005
Newman-ΔuhpT	S. aureus Newman with deletion of uhpT	This study
Newman-ΔglpT	S. aureus Newman with deletion of glpT	This study
Newman-ΔuhpT&glpT	S. aureus Newman with deletion of both uhpT and glpT	This study
Newman-ΔuhpT+pRB473-uhpT	Newman-ΔuhpT complemented with uhpT by plasmid pRB473	This study
Newman-ΔglpT+pRB473-glpT	Newman-ΔglpT complemented with glpT by plasmid pRB473	This study
SA2+pRB473-uhpT	SA2 complemented with uhpT by plasmid pRB473	This study
SA2+pRB473-glpT	SA2 complemented with glpT by plasmid pRB473	This study
SA94+pRB473-uhpT	SA94 complemented with uhpT by plasmid pRB473	This study
SA94+pRB473-glpT	SA94 complemented with glpT by plasmid pRB473
SA30+pRB473-uhpT	SA30 complemented with uhpT by plasmid pRB473
SA30+pRB473-glpT	SA30 complemented with glpT by plasmid pRB473	This study
Plasmids
pKOR1	E. coli – S. aureus shuttle vector; Ampr in E. coli; Cmr in S. aureus	Bae and Schneewind, 2005
pKOR1-ΔuhpT	pKOR1 with deletion mutation of uhpT	This study
pKOR1-ΔglpT	pKOR1 with deletion mutation of glpT	This study
pRB473	E. coli – S. aureus shuttle vector; Cmr in S. aureus	Wang et al., 2015
pRB473-uhpT	pRB473 ligated with uhpT	This study
pRB473-glpT	pRB473 ligated with glpT	This study