Figure 1.

Maturation of the Spontaneous Tectal Activity in Intact and Retinal Input Deprived Larvae

(A) Raster plot examples of the spontaneous significant Ca²⁺ events of intact larvae at 2.5, 3, 5, and 8 dpf (left column, ns = 880, 1,012, 927 and 810 neurons, respectively) and an enucleated larva at 3, 5, and 8 dpf (right column, ns = 980, 882, and 879 neurons, respectively). The rasters represent a period of 2,000 s out of ∼1 hr of recording sessions. Gray scale: amplitude of the Ca²⁺ events. The percentage of active neurons at each time point is represented below each raster plot.

(B–D) Developmental dynamics of the average frequency (B), duration (C), and relative fluorescence variation (D; ΔF/F) of the spontaneous Ca²⁺ events.

(E) Average frequency of the synchronous spontaneous Ca²⁺ events.

(F) Average distance between the distributions of the pairwise correlation coefficients and their respective random surrogate versions for each developmental stage in intact and enucleated larvae, quantified as JSD. Blue indicates intact larvae, and red indicates enucleated larvae.

^∗: p < 0.05; ^∗∗: p < 0.01; ^∗∗∗: p < 0.001. Error bars indicate SEM.