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. 2017 May 2;19(5):1022–1032. doi: 10.1016/j.celrep.2017.04.025

Figure 2.

Figure 2

ATR Activation Is Independent of Viral Genome Uncoating

(A) Immunoblot analysis reveals that cycloheximide (CHX) and the proteasome inhibitor MG-132, but not AraC, inhibit both RPA Ser33 phosphorylation and late viral protein expression (F13).

(B) Quantitative immunofluorescence analysis reveals that MG-132 inhibits vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity (green).

(C) Quantitative immunofluorescence analysis reveals that loss of D5 does not inhibit vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity or expression of I3.

All error bars represent SEM from three independent experiments in which a minimum of 200 cells were counted, with ∗p < 0.05, ∗∗p < 0.005, and ∗∗∗p < 0.001. Scale bars, 20 μm.