Figure 5.

Viral Genomes Recruit Replisome-Associated RPA2 and Activate ATR

(A) Immunofluorescence analysis of cells infected for 4 hr reveals a reduced incorporation of CldU (green) into developing viral factories in cell treated with siRNA against ATR, RPA2, Rhino, and INTS7.

(B) Rhino is re-localized from the nucleus to the cytoplasm after infection, while INTS7 co-localizes with viral DNA factories 8 hr post-infection (arrowheads).

(C) Immunoblot analysis of GFP-Trap pull-downs on cells infected with GFP-H5 virus for 5 hr.

(D) Knockdown of ATR and TOPBP1 reduces incorporation of CldU (green) into developing viral factories.

(E) Immunoblot analysis of GFP-Trap pull-downs on cells infected with GFP-H5 virus for 5 hr in the presence of AraC or after lysates were treated with DNase I.

Scale bars, 20 μm.