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. 2017 Jan 5;36(4):536–548. doi: 10.15252/embj.201695028

Figure 3. Expression of catalytically inactive MarPS343A in MarP‐deficient cells impairs growth and causes the formation of chains.

Figure 3

  1. Mtb growth curve in Sauton's medium at pH 7.2. Mtb WT and ΔmarP expressing marPS343A from an Atc‐inducible promoter; 1 μg/ml of Atc was added at day 6 (closed symbols). Data are means of triplicate cultures and error bars represent SD.
  2. Growth of Msm in 7H9 medium. Msm WT and ΔmarP expressing marPS343A from an Atc‐inducible promoter; 200 ng/ml of Atc was added after 3 h of incubation (closed symbols).
  3. Representative images of Msm ΔmarP expressing marPS343A from an Atc‐inducible promoter, with no Atc (2 panels on the left) or 200 ng/ml of Atc (2 panels on the right). Arrows show examples of bacteria that formed chains. Scale bars, 1 μm.
Data information: Data in panels (A, B, and C) are representative of three independent experiments. See also Appendix Fig S3.