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. 2017 Jan 5;36(4):536–548. doi: 10.15252/embj.201695028

Figure 6. Msm ΔripA forms chains during acidic stress.

Figure 6

  1. Boxplot of the lengths (μm) of Msm WT, ΔripA, and ΔripA/ripA smeg incubated for 24 h in Sauton's medium at pH 7.2 or pH 4.5, measured in three independent experiments. Lower and upper whiskers extend to the 10th and 90th percentiles, respectively. The middle bar represents the median and the lower and upper box limits are the 25th and 75th percentiles. The P‐values have been computed using the Ranksum test adjusted for multiple testing. For Msm WT pH 7.2, n = 335, 213, 202; pH 4.5, n = 395, 216, 180. For ΔripA pH 7.2, n = 367, 214, 207; pH 4.5, n = 404, 149, 113. For ΔripA/ripA smeg pH 7.2, n = 323, 281, 181; pH 4.5, n = 350, 186, 192. n indicates the number of cells in experiments 1, 2, and 3. Adjusted P‐values compared to WT pH 7.2: ΔripA pH 7.2 P = 8.4 × 10−5 ; ΔripA/ripA smeg pH 7.2, P = 6.7 × 10−3. Adjusted P‐values compared to WT pH 4.5: ΔripA pH 4.5, P = 1.3 × 10−15; ΔripA/ripA smeg pH 4.5, P = 7.7 × 10−4.
  2. Representative images of Msm WT, ΔripA, and ΔripA/ripA smeg incubated in Sauton's medium at pH 7.2 or at pH 4.5 containing 1 mM NADA for 24 h. Arrows show examples of bacteria that formed chains. Scale bars, 1 μm.
  3. Quantification of the number of bacteria presenting two, three or four septa for Msm WT, ΔripA, and ΔripA/ripA smeg after 24‐h incubation at pH 7.2 or pH 4.5 from three independent experiments. Error bars represent SEM. P‐values were determined using a logistic regression model and adjusted for multiple comparisons.