Figure 4. Autophagy flux is unaffected by sensors.

• A–C
  Analysis of autophagy flux in stable inducible HeLa cell lines expressing mCherry fusion proteins of indicated ATG8 sensor upon doxycycline (DOX) induction: Autophagy flux is monitored in basal conditions (A), upon starvation (EBSS) (B), or after autophagy induction by Torin 1 (C) indirectly via lipidation of LC3B and turnover of the autophagy receptor p62.
• D
  FACS‐based mitophagy flux analysis performed in HeLa cell line stably expressing HA‐Parkin, mt‐mKeima, and a GFP fusion of the indicated sensor. Cells were treated with OAQ (oligomycin, antimycin, Q‐VD) for the indicated times and analyzed by FACS for lysosomal‐positive mt‐mKeima (561 nm). The average of three independent experiments is presented. Representative FACS data from experiments (GFP control and AS3_67) are shown on the lower panels. Error bars indicate standard deviation. Significance calculation: two‐way ANOVA of one‐way ANOVA. n.s. = not significant. *** indicates P‐value of 0.0001.

Source data are available online for this figure.