Figure 4.

Paraquat treatment changes the distribution of reactive oxygen species in ahr1 and wav2-1 mutants. Root seedlings of Col-0 (A), ahr1 (B), Ler (C), and wav2-1 (D) were grown in the vertical position in the presence of paraquat (PQ). Each panel shows root tips in optical bright field (left) and fluorescent images (right). Roots of the ahr1 mutant and Col-0 had considerably higher fluorescence than the wav2-1 mutant and Ler (A-B). These genotypes (Ler and wav2-1) had a significantly reduced fluorescence signals under thigmotropic stimulus when in the presence of PQ (C-D) (see data in Supplemental Table 1). Scale bar = 200 µm.