Evolutionary dynamics in two-strain communities with BC (squares, dotted line), PA wild type (circles, solid line) and PA cheat (triangles, dashed line) in low and highly structured iron-limited CAA medium. Co-existence of BC and PA wild type is not possible, neither in unstructured (a) nor structured medium (b). BC becomes the dominant strain in competition with the PA cheat after 2 or 3 days regardless of spatial structure (c, d). The PA cheat outcompetes the PA wild type only in medium with low spatial structure (e) but not in highly structured medium (f). Symbols depict individual data points (from four replicates), whereas lines represent mean strain frequencies.