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. 2017 Jan 20;11(5):1245–1260. doi: 10.1038/ismej.2016.203

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Copyright © 2017 International Society for Microbial Ecology

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Phylogenetic tree of [NiFe]-hydrogenases constructed with full-length enzymes from small HydA (a) and large HydB (b) subunits of subgroup representatives. Based on the report Vignais and Billoud (2007), the alignment was made with Clustal W584 and MacVector 11.1.2. Trees were computed with PhyML586 using the bootstrap procedure with 1000 replicates and bootstrap values of more than 700 (70%) are displayed as percentages close to the corresponding nodes. The nodes are displayed so that the corresponding small and large subunits can be read in the same top-down order. Branch lengths along the horizontal axis reflect the degree of relatedness of the sequences (20%).