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. 2017 May 19;37(3):BSR20170150. doi: 10.1042/BSR20170150

Figure 1. Four strategies for gating dMo in 8-color MFC.

Figure 1

The cells were stained for CD3, CD56, CD16, CD4, CD8, IFN-γ, and IL-4 with a Vt dye. The dMo are shown separately by an FSC/SSC gate (A) and an FSC/Vt gate (B) with a central scale or by an FSC/SSC gate (C), and an FSC/Vt gate (D) with an extended scale. The next gating steps were the same for each strategy except a bisector gate was added for the events obtained from the FSC/SSC gate (A,C) to exclude the dead cells. Decidual T, NK, NKT-like, Th and Tc cells and their type-1 or type-2 subsets are delineated by all these strategies. The events obtained from the FSC/Vt gate were reviewed by the FSC/SSC gate with both scales (B,D), which showed that there were more events in the FSC/Vt gate than in the FSC/SSC gate, especially with the extended scale.