Table 4. Subpopulation proportions delineated by FSC/Vt (central) and FSC/Vt (extended) strategies in normal pregnancy and URM.
Marker | Cell | FSC/Vt (central) | FSC/Vt(extend) | ||
---|---|---|---|---|---|
Normal pregnancy | URM | Normal pregnancy | URM | ||
CD3+CD56– | T | 25.4 ± 2.6 | 25.5 ± 2.9 | 19.5 ± 2.1 | 21.8 ± 2.8 |
CD3–CD56brightCD16– | dNK | 41.9 ± 4.2 | 48.3 ± 4.0 | 45.0 ± 3.4 | 49.6 ± 3.8 |
CD3–CD56brightCD16–/ CD3+CD56– | dNK/T | 1.5 ± 0.1 | 2.0 ± 0.4 | 2.2 ± 0.1 | 2.2 ± 0.1 |
CD3+CD56+ | NKT-like | 7.4 ± 1.1 | 6.1 ± 1.4 | 7.3 ± 0.7 | 6.1 ± 1.5 |
CD3+CD4+CD8– | Th | 55.5 ± 2.0 | 49.8 ± 5.9 | 52.6 ± 2.0 | 47.3 ± 5.9 |
CD3+CD4–CD8+ | Tc | 27.0 ± 1.5 | 25.7 ± 1.9 | 30.2±1.5 | 26.7 ± 1.9 |
CD3+CD4+CD8–/ CD3+CD4–CD8+ | Th/Tc | 2.1 ± 0.1 | 2.1 ± 0.2 | 2 ± 0.2 | 1.9 ± 0.2 |
CD3+CD4+CD8–IFN-γ+IL-4– | Th1 | 38.5 ± 2.5 | 46.8 ± 1.8 | 35.4 ± 1.9 | 46.8 ± 1.8 |
CD3+CD4+CD8–IFN-γ–IL-4+ | Th2 | 0.6 ± 0.1 | 0.5 ± 0.1 | 0.5 ± 0.1 | 1.1 ± 0.3 |
CD3+CD4+CD8–IFN-γ+IL-4+ | Th DP* | 2.9 ± 0.5 | 4.5 ± 1.0 | 2.2 ± 0.3 | 6.6 ± 1.2 |
CD3+CD4+CD8–IFN-γ+IL-4– /CD3+CD4+CD8–IFN-γ–IL-4+ | Th1/Th2 | 111.8 ± 25.9 | 133.9 ± 14.7 | 118.3 ± 25.7 | 75.5 ± 15.8 |
CD3+CD4–CD8+IFN-γ+IL-4– | Tc1 | 50.1 ± 2.5 | 63.5 ± 2.4 | 44.0 ± 2.6 | 63.9 ± 2.7 |
CD3+CD4–CD8+IFN-γ–IL-4+ | Tc2 | 1.5 ± 0.4 | 0.9 ± 0.3 | 3.0 ± 0.9 | 0.9 ± 0.3 |
CD3+CD4–CD8+IFN-γ+IL-4+ | Tc DP* | 2.4 ± 0.6 | 2.7 ± 0.6 | 3.0 ± 0.9 | 3.3 ± 0.7 |
CD3+CD4−CD8+IFN-γ+IL-4− /CD3+CD4– CD8+IFN-γ–IL-4+ | Tc1/Tc2 | 134.7 ± 32.4 | 89.2 ± 9.2 | 88.9 ±3 7.6 | 87.0 ± 10.1 |
CD3–CD56brightCD16–IFN-γ+IL-4– | dNK1 | 4.3 ± 0.9 | 9.5 ± 2.5 | 3.1 ± 0.7 | 13.0 ± 2.9 |
CD3–CD56brightCD16–IFN-γ–IL-4+ | dNk2 | 0.7±0.2 | 1.3±0.4 | 0.2±0.1 | 4.7 ± 1.7 |
CD3–CD56brightCD16–IFN-γ+IL-4+ | dNK DP* | 0 | 0.1±0.02 | 0 | 0.6 ± 0.2 |
CD3–CD56brightCD16–IFN-γ+IL-4– /CD3–CD56brightCD16–IFN-γ–IL-4+ | dNk1/dNK2 | 25.4 ± 11.4 | 24.1 ± 13.0 | 32.0 ± 10.1 | 7.1 ± 2.4 |
*DP represents double positive cell.