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. 2017 Jan 2;6(1):1–9. doi: 10.1093/gigascience/giw004

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© The Author 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — k-means clustering for differentially expressed genes and expression patterns. (A) Eight expression patterns are shown on the left. Heat map shows the relative expression levels of each transcript (rows) in each sample (column). Normalized fragments per kilobase of exon per million fragments (FPKMs) calculated by Cuffdiff2 were log₂-transformed and then median-centered by transcript. Heatmap was drawn based on clustering results. Red color represents higher expression; green represents lower expression. Note: red asterisks (*) on the left side of the figure indicate that expression at the corresponding stage is higher than the average expression level. Abbreviations: emb: embryo; 1in: 1^st instar nymph; 2in: 2^nd instar nymph; 3in: 3^rd instar nymph; 4in: 4^th instar nymph; 5in: 5^th instar nymph; 5d: 5-day-old adult; 10d: 10-day-old adult. (B) The average of log₂-transformed FPKM corresponding genes in each pattern.