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. 2017 May 19;12(5):e0175918. doi: 10.1371/journal.pone.0175918

Fig 2. The Abcf1 promoter is highly expressed during early embryogenesis.

Fig 2

A) Blastocysts were isolated from 3.5 dpc female mice and stained with X-gal. Three separate phenotypes were observed: non-stained (Abcf1+/+), intermediate-stained (Abcf1+/-) and darkly stained (Abcf1-/-), which directly corresponded to their genotypes and to the gene copy number of the β-geo reporter. The darkly stained blastocysts also appeared to be slightly oblong in shape compared with the medium-stained embryos and lacked a zona pellucida. Images were taken using a 20× lens. B) To distinguish between homozygous-knockout and heterozygous blastocysts, the blastocysts from A were genotyped by PCR to confirm the genotypes. The top image shows the presence of the knockout allele in both blastocysts. The bottom image (taken using an QIAxcel Capillary Electrophoresis machine at Biomedical Research Centre, UBC) shows the presence of the wild type allele only in the heterozygous blastocyst.