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. 2017 May 19;12(5):e0175918. doi: 10.1371/journal.pone.0175918

Fig 6. Quantitative representation of X-gal staining.

Fig 6

Image quantification was done using MatLab (MathWorks, Apple Hill Drive, MA, USA), as described in the material and methods section. 7 animals each of control (Abcf1+/+; no β-geo) and heterozygotes (Abcf1+/-; expressing β-geo) were sacrificed, tissues were extracted and staining intensities were measured. The error bar represents the standard deviation. Percentage 1–10% is characterized as light or faint staining, 10–40% as intermediate staining and greater than 40% as intense staining.