Fig. 2.

SlincR and sox9b-eGFP are expressed in adjacent and overlapping tissues through multiple stages of development. Lateral (A) and ventral (B) views of dual immunohistochemistry and in situ hybridization samples targeting sox9b-eGFP (green) and slincR (red) in sox9b-eGFP reporter fish at 36 and 48 hpf (n = 6–10 embryos). The fish are outlined with a white line, slincR expression is outlined with a dotted white line, and the eye is labeled the letter e. The white rectangle represents the magnified area depicted in the inset. Both scale bars represent 100 µm. All experiments were independently repeated a minimum of two times.