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. 2017 May 19;12(5):e0177921. doi: 10.1371/journal.pone.0177921

Fig 2. Whole-lobe multiwavelength cell scoring of the remaining cardiac lobe after left pneumonectomy.

Fig 2

A, B) The positively curved posterior pleural surface stained for α-smooth muscle actin (SMA)(SMA = red, bar = 200um). C) Extending previous observations of SMA expression in the first 3 days after pneumonectomy [11], the most reproducible expression was on a postoperative day 3 (POD 3). D) SMA expression after pneumonectomy (PNX) was compared to conditions controlling for pleural deformation: sham thoracotomy (Sham), pneumonectomy and plombage (PNX+PLB), pneumonectomy and phrenic nerve transection (PNX+PNT)(N = 3 each condition). SMA expression after pneumonectomy mice significantly higher than in the three control conditions (p < .01). The difference was greater when the analysis was restricted to the posterior curvature of the cardiac lobe (p < .01). There was no difference between the control conditions (p>.05).