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. 2017 May 19;12(5):e0177542. doi: 10.1371/journal.pone.0177542

Table 1. Biomarkers detected in the total organic extract of the Frutexites-like biofilm from Äspö tunnel.

Compound class RT Peak No. MW Formula Compound %
19.62 1 168 C16H30O Hexadecenal 0.05
20.1 2 Unknown compound 0.06
Fatty acids 22.92 3 300 C17H36O2Si C14:0 TMS 0.03
24.18 4 314 C18H38O2Si C15:0 i TMS 0.09
24.34 5 314 C18H38O2Si C15:0 ai TMS 0.04
24.47 6 312 C18H36O2Si C15:1 TMS 0.10
24.91 7 314 C18H38O2Si C15:0 TMS 0.08
26.09 8 328 C19H40O2Si C16:0 i TMS 0.02
26.25 9 326 C19H38O2Si C16:1 TMS 0.15
26.37 10 326 C19H38O2Si C16:1 TMS 0.43
26.48 11 326 C19H38O2Si C16:1 TMS 0.19
26.58 12 326 C19H38O2Si C16:1 TMS 0.16
26.8 13 328 C19H40O2Si C16:0 0.39
27.61 14 342 C20H42O2Si 10MeC16:0 TMS 0.07
27.95 15 342 C20H42O2Si C17:0 i TMS 0.04
29.99 16 354 C21H42O2Si C18:1 TMS 0.16
30.36 17 356 C21H44O2Si C18:0 TMS 0.08
32.21 18 370 C22H46O2Si C19:0 TMS 0.14
34.71 19 384 C23H48O2Si C20:0 TMS 0.21
39.18 20 410 Squalene 0.86
41.85 21 Unknown compound 1.87
44.34 22 Unknown compound 1.99
Sterols 43.36 23 458 C30H54OSi Cholest-5-en-3β-ol TMS (cholesterol) 5.59
43.86 24 470 C31H54OSi 24-Methylcholesta-5,22-dien-3-β-ol TMS (ergostan5,22-dienol) 1.88
44.57 25 470 C31H54OSi 24-Methylcholesta-5,7-dien-3-β-ol TMS (ergostan5,7-dienol) 1.19
44.65 26 472 C33H54OSi 24-Methylcholest-5-en-3β-ol TMS (campesterol) 1.04
44.98 27 484 C32H56OSi 24-Ethylcholest-5, 22-en-3β-ol TMS (sitostadienol) 0.59
45.69 28 486 C32H58OSi 24-Ethylcholest-5-en-3β-ol TMS (sitosterol) 4.01
45.83 29 488 C32H60OSi 24-Ethylcholestan-3β-ol TMS (sitostanol) 0.61
45.88 30 484 C32H56OSi 24-Ethylcholest-5-en-3β-ol TMS (sitosterol) 1.29
50.42 31 504 Unknown steroid 5.22
Hopanoids 42.73 32 410 C30H50 Hop17(21)-ene 1.57
43.72 33 384 C28H48 17β(H),18α(H),21β(H)-Bisnorhopane 1.02
45.44 34 410 C30H50 Hop22(29)-ene (Diploptene) 9.34
45.55 35 410 C30H50 Hop21(22)-ene 4.35
47.82 36 428 C30H52O Diplopterol 11.35
48.44 37 Unknown hopanoid 2.38
48.52 38 Unknown compound 2.12
48.89 39 Unknown hopanoid 3.57
49.77 40 440 C32H56 17ß(H),21ß(H)-Bishomohopane 1.89
51.19 41 454 C32H54O 17ß(H),21ß(H)-Bishomohopanone 13.25
52.09 42 528 C34H60O2Si 17ß(H),21ß(H)-Homohopanoic acid-TMS 5.14
56.83 43 Unknown hopanoid 2.15
57.71 44 Unknown hopanoid 2.12
61.27 45 616 C38H72O2Si2 17ß(H),21ß(H)-Bishomohopandienol TMS 4.97
69.65 46 834 C47H94O4Si4 17ß(H),21ß(H)-Bacteriohopanetetrol TMS 6.16
Sum 100.00