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. 2017 May 19;12(5):e0177736. doi: 10.1371/journal.pone.0177736

Fig 1. Generation of human IgG1 and IgG3 TA99 mAb with histidine-arginine rearrangements in Fc domains at amino acid position 435.

Fig 1

Human IgG1 was mutated to contain an arginine at position 435 (IgG1 H435R), whereas the arginine at position 435 in human IgG3 was changed to histidine (IgG3 R435H). (A) Specific anti-human IgG1 or (B) anti-human IgG3 ELISA confirmed the correct isotype of mAbs. Staining of B16F10-gp75 with (C) different human TA99 mAb and (D) mouse TA99 IgG2a confirmed binding to surface gp75 and equal binding efficiency to gp75 of all human TA99 mAb. Concentration curves of human IgG1 and IgG3 mAb (E) and mouse IgG2a TA99 (F) on B16F10-gp75. Of note, scales of human (E) and mouse (F) antibodies are different.