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. 2017 May 19;12(5):e0178013. doi: 10.1371/journal.pone.0178013

Fig 12. Treatment with a PDE4B inhibitor reduced neuronal loss in the hippocampal CA3 region at 2 months post-injury.

Fig 12

Representative images of the ipsilateral CA3 region of the hippocampus immunolabeled for mature neurons using NeuN in (A) sham, (B) vehicle-treated and (C) A33-treated TBI animals. Representative images at -5.3 mm bregma, scale bar 250 μm. (D) Quantification of NeuN+ cells in the ipsilateral and contralateral CA3 region of the hippocampus. Vehicle and A33-treated TBI animals had significantly reduced NeuN+ cells in the ipsilateral hippocampal CA3 region as compared to sham animals. A33 treatment partially rescued neuronal loss in the ipsilateral hippocampal CA3 region as compared to vehicle-treated TBI animals. (main effect of treatment: F(2, 64) = 8.977, p = 0.0004; main effect of region (ipsi vs. contra): F(1, 64) = 18.45, p<0.0001; interaction of treatment x region: F(2, 64) = 13.38, p<0.0001.) Mean ± SEM, n = 10-13/group, **p<0.01, ***p<0.001 vs. Ipsi/Contra Sham, #p<0.01, ##p<0.001 vs. Ipsi TBI+Vehicle, two-way ANOVA with post-hoc Student-Newman-Keuls.