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. 2017 Apr 7;6:e21231. doi: 10.7554/eLife.21231

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© 2017, Hockman et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 1. — (a) Schematic of a 7–8 dpf zebrafish; dotted line indicates section plane in b-f’. (b) Hematoxylin and eosin staining at 7-dpf reveals gill filaments branching from branchial arch cartilages, and the orobranchial cavity. Dashed box indicates approximate region in c,g. (c–d’) In 7-dpf Tg(-28.5sox10:cre);Tg(ef1a:loxP-DsRed-loxP-EGFP) zebrafish, GFP labels neural crest-derived branchial arch cartilage and mesenchyme, but not NECs in the gill filaments (identified by immunoreactivity for serotonin, 5-HT; arrowheads). (e–f’) Horizontal section through the gills of a 20-dpf Tg(-4.9sox10:creER^T2);Tg(βactin:loxP-SuperStop-loxP-DsRed) zebrafish. DsRed (brown precipitate) labels neural crest-derived gill pillar cells, but not NECs (arrowheads; inverted fluorescent image overlaid on bright-field image). (g–h’) In 8-dpf Tg(crestin:creER^T2);Tg(-3.5ubi:loxP-GFP-loxP-mCherry) zebrafish, mCherry labels gill pillar cells but not NECs (arrowheads). 5-HT, serotonin; Bac, branchial arch cartilage; Gf, gill filament; La, lamellae; Nt, neural tube; Obc, orobranchial cavity. Scale-bars: 50 μm in b,c,e,g; 25 μm in d,f,h.

DOI: http://dx.doi.org/10.7554/eLife.21231.003

Figure 1—figure supplement 1. — (a) Schematic of a 7–8 dpf zebrafish; dotted line indicates section plane in b-f’. (b) Hematoxylin and eosin staining at 7-dpf reveals gill filaments branching from branchial arch cartilages, and the orobranchial cavity. Dashed box indicates approximate region in c,g. (c–d’) In 7-dpf Tg(-28.5sox10:cre);Tg(ef1a:loxP-DsRed-loxP-EGFP) zebrafish, GFP labels neural crest-derived branchial arch cartilage and mesenchyme, but not NECs in the gill filaments (identified by immunoreactivity for serotonin, 5-HT; arrowheads). (e–f’) Horizontal section through the gills of a 20-dpf Tg(-4.9sox10:creER^T2);Tg(βactin:loxP-SuperStop-loxP-DsRed) zebrafish. DsRed (brown precipitate) labels neural crest-derived gill pillar cells, but not NECs (arrowheads; inverted fluorescent image overlaid on bright-field image). (g–h’) In 8-dpf Tg(crestin:creER^T2);Tg(-3.5ubi:loxP-GFP-loxP-mCherry) zebrafish, mCherry labels gill pillar cells but not NECs (arrowheads). 5-HT, serotonin; Bac, branchial arch cartilage; Gf, gill filament; La, lamellae; Nt, neural tube; Obc, orobranchial cavity. Scale-bars: 50 μm in b,c,e,g; 25 μm in d,f,h.

DOI: http://dx.doi.org/10.7554/eLife.21231.003