Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 7;6:e21231. doi: 10.7554/eLife.21231

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017, Hockman et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 2. — (a) 7-dpf tfap2a^mob;foxd3^mos zebrafish lack all neural crest derivatives, including melanophores and jaw skeleton (arrowhead). Dotted lines: section planes in b-e. (b,c) At 7-dpf, tfap2a^mob;foxd3^mos orobranchial epithelium retains innervated serotonergic (5-HT⁺) cells (putative NECs), identified by cytoplasmic serotonin surrounded by a ring of HNK1 epitope-immunoreactive neurites. (d,e) At 7-dpf, putative NECs (arrowheads) persist in tfap2a^mob;foxd3^mos zebrafish in the region ventral to the orobranchial cavity where the pharyngeal arches would be located in wild-type fish. (NB The hypothalamus [Hyp] extends caudally beneath the midbrain and rostral hindbrain, and often separates from the overlying brain on sections, as here.) (f) The mean number per 7-dpf larva of putative NECs in the orobranchial epithelium does not differ between tfap2a^mob;foxd3^mos (98.0 ± 49.7 s.d.; n = 3) and wild-type zebrafish (81.3 ± 24.0 s.d.; n = 3) (p=0.63, unpaired two-tailed Student’s t-test). All such cells in the orobranchial epithelium were counted for each embryo. Error bars indicate s.d. (g–i) Wild-type sibling at 7-dpf. Dotted line: section plane in h-i. Putative NECs are present in the orobranchial epithelium (arrowheads; dashed box in i, magnified without DAPI in top right corner). 5-HT, serotonin; Gf, gill filament; Hyp, hypothalamus; Nt, neural tube; Obc, orobranchial cavity. Scale-bars: 50 μm in b,d,h; 25 μm in c,e,i.

DOI: http://dx.doi.org/10.7554/eLife.21231.005

Figure 2—figure supplement 1. — (a) 7-dpf tfap2a^mob;foxd3^mos zebrafish lack all neural crest derivatives, including melanophores and jaw skeleton (arrowhead). Dotted lines: section planes in b-e. (b,c) At 7-dpf, tfap2a^mob;foxd3^mos orobranchial epithelium retains innervated serotonergic (5-HT⁺) cells (putative NECs), identified by cytoplasmic serotonin surrounded by a ring of HNK1 epitope-immunoreactive neurites. (d,e) At 7-dpf, putative NECs (arrowheads) persist in tfap2a^mob;foxd3^mos zebrafish in the region ventral to the orobranchial cavity where the pharyngeal arches would be located in wild-type fish. (NB The hypothalamus [Hyp] extends caudally beneath the midbrain and rostral hindbrain, and often separates from the overlying brain on sections, as here.) (f) The mean number per 7-dpf larva of putative NECs in the orobranchial epithelium does not differ between tfap2a^mob;foxd3^mos (98.0 ± 49.7 s.d.; n = 3) and wild-type zebrafish (81.3 ± 24.0 s.d.; n = 3) (p=0.63, unpaired two-tailed Student’s t-test). All such cells in the orobranchial epithelium were counted for each embryo. Error bars indicate s.d. (g–i) Wild-type sibling at 7-dpf. Dotted line: section plane in h-i. Putative NECs are present in the orobranchial epithelium (arrowheads; dashed box in i, magnified without DAPI in top right corner). 5-HT, serotonin; Gf, gill filament; Hyp, hypothalamus; Nt, neural tube; Obc, orobranchial cavity. Scale-bars: 50 μm in b,d,h; 25 μm in c,e,i.

DOI: http://dx.doi.org/10.7554/eLife.21231.005