Video 2. Putative NECs in the skin of embryonic zebrafish are not neural crest-derived.
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DOI: 10.7554/eLife.21231.008
Z-stack movie at higher powerthan Video 1, showing a whole-mount view of the eye region of a 3-dpfTg(crestin:creERT2);Tg(-3.5ubi:loxP-GFP-loxP-mCherry)embryo, immunostained for serotonin and mCherry. Serotonergic cells (arrowheads) are scattered in the epidermis over the eye, as reported (Coccimiglio and Jonz, 2012), but none is mCherry-positive, i.e., neural crest-derived. A 3-dimensional rendering for the eye is shown in Figure 2—figure supplement 1.