Figure 5. Genetic determinants of adaptation to ramped increases in H₂O₂.

Quantification of mean growth rate upon exposure to a linear ramp (δ = 1.1 μM/min) starting at t = 100 min in various genotypes. (A) Illustration of the H₂O₂ ramp experiment indicating the timing of the measurements. (B) Stress response and membrane permeability mutants. (C) Details of the ramp experiment in the Δyap1 mutant. The dashed blue lines indicate the adaptation threshold obtained in step experiments (Figure 1G). (D) Yap1 effectors mutants. (E) Step experiment performed with Yap1 effectors mutants exposed to 0.4 mM H₂O₂. (F) Prxs mutants. (G) Schematic of a negative feedback control showing the essential role of Prxs in the H2O2 homeostasis. (H) Mutants affecting the peroxidatic cycle of Prxs. Error bars are SEM (N > 100). See also Figure 5—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.23971.020

Figure 5—figure supplement 1. Quantification of Tsa1 expression from the ACT1 promoter.

(A) Phase and GFP fluorescence image samples of cells carrying a Tsa1-GFP fusion (top) or an ACT1pr-TSA1-GFP fusion (bottom). (B) Quantification of mean cell cytoplasmic fluorescence for strains described in (A) Error bars are SEM (N > 100).

DOI: http://dx.doi.org/10.7554/eLife.23971.021