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. 2017 May 10;6:e26886. doi: 10.7554/eLife.26886

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© 2017, Wang et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 1. — (A) Immunostaining images show NMJs of w¹¹¹⁸, leon²/19-2, leon¹/19-2 and GFP-leon-GR, leon¹/19-2 for HRP (red) and Dlg (green). (B–D) Bar graphs show means ± SEM (standard error of mean) of bouton numbers (B), branch lengths (C) and muscle areas (D, quantified by phalloidin staining, not shown). (E and G) Immunostaining images co-stained by HRP (red) and Dlg (green) show transgene rescue of leon¹/19-2 NMJs by UAS-leon or UAS-ED-leon driven by postsynaptic 24B-GAL4 (E) or presynaptic D42-GAL4 (G). (F and H) Bar graphs show means ± SEM of bouton numbers in postsynaptic or presynaptic rescue. Scale bars, 20 μm. All data were compared to controls unless specifically indicated by brackets, with n.s. indicating no significance, * for p<0.05, and *** for p<0.001 according to Student’s t tests. The detail statistic numbers also see Supplementary file 1.

DOI: http://dx.doi.org/10.7554/eLife.26886.002

Figure 1—figure supplement 1. — (A) Immunostaining images show NMJs of w¹¹¹⁸, leon²/19-2, leon¹/19-2 and GFP-leon-GR, leon¹/19-2 for HRP (red) and Dlg (green). (B–D) Bar graphs show means ± SEM (standard error of mean) of bouton numbers (B), branch lengths (C) and muscle areas (D, quantified by phalloidin staining, not shown). (E and G) Immunostaining images co-stained by HRP (red) and Dlg (green) show transgene rescue of leon¹/19-2 NMJs by UAS-leon or UAS-ED-leon driven by postsynaptic 24B-GAL4 (E) or presynaptic D42-GAL4 (G). (F and H) Bar graphs show means ± SEM of bouton numbers in postsynaptic or presynaptic rescue. Scale bars, 20 μm. All data were compared to controls unless specifically indicated by brackets, with n.s. indicating no significance, * for p<0.05, and *** for p<0.001 according to Student’s t tests. The detail statistic numbers also see Supplementary file 1.

DOI: http://dx.doi.org/10.7554/eLife.26886.002