Figure 7. TCF7L1 promotes cell migration in SCC cells independently of β-catenin binding.
The human skin SCC cell lines SCC13 and SRB12 were transduced with tet-inducible Tcf7l1 or Tcf7l1 deletion mutants. After drug selection, the transduced cells were treated with doxycycline for 48 hr and their migratory ability was measured using the Boyden chamber-based cell migration assay. Bar denotes 200 µm. (A) Representative images of migrated SCC13 cells stained with crystal violet. (B–C) Quantification of migrated SCC cells, SCC13 (B) or SRB12 (C), per 5X field. Data are presented as mean ± s.d. *p<0.05, **p<0.01, ***p<0.001 (One-way ANOVA with Dunnett’s post-hoc test).