TABLE I.
Quantitative Microsphere Hybridization Probes Utilized in the Study of Prader-Willi Syndrome
| Probe name | Chromosome location (March 2006) | Secondary structure analysis |
|---|---|---|
| PWS19.4 Mb | chr15:19,412,113–19,412,168 | dG = −7.48,dH = −89.30,dS = −263.81,Tm = 65.4 |
| D15S541 | chr15:20,360,015–20,360,075 | dG = −4.19,dH = −80.40,dS = −235.83,Tm = 67.8 |
| GCP5 | chr15:20,402,365–20,402,429 | dG = −0.97,dH = −40.30,dS = −121.71,Tm = 58.0 |
| NIPA1 | chr15:20,637,672–20,637,733 | dG = −7.00,dH = −100.60,dS = −301.79,Tm = 60.2 |
| chr15:21,263,422 | chr15:21,263,422–21,263,482 | dG = −2.99,dH = −89.00,dS = −277.32,Tm = 47.8 |
| chr15:21,402,665 | chr15:21,402,665–21,402,725 | dG = −3.68,dH = −85.60,dS = −253.50,Tm = 64.5 |
| D15S11 | chr15:21,625,574–21,625,639 | dG = −5.20,dH = −105.00,dS = −308.83,Tm = 66.8 |
| D15S63 | chr15:22,581,253–22,581,317 | dG = −1.53,dH = −70.70,dS = −214.05,Tm = 57.1 |
| D15S128 | chr15:22,682,940–22,682,995 | dG = −6.81,dH = −99.30,dS = −298.21,Tm = 59.8 |
| AS-SRO | chr15:22,717,169–22,717,238 | dG = −0.37,dH = −25.00,dS = −76.22,Tm = 54.9 |
| IC3 | chr15:22,717,771–22,717,826 | dG = −5.40,dH = −63.90,dS = −188.62,Tm = 65.6 |
| IC2 | chr15:22,721,121–22,721,175 | dG = −5.75,dH = −63.20,dS = −185.23,Tm = 68.0 |
| IC1 | chr15:22,732,614–22,732,670 | dG = −2.45,dH = −55.10,dS = −169.76,Tm = 51.4 |
| IC | chr15:22,736,900–22,736,965 | dG = −1.45,dH = −50.30,dS = −151.17,Tm = 59.6 |
| SNRPN exon1 | chr15:22,751,254–22,751,319 | dG = −7.31,dH = −132.30,dS = −403.00,Tm = 55.1 |
| PWS-SRO | chr15:22,751,461–22,751,525 | dG = −4.82,dH = −62.40,dS = −178.18,Tm = 77.1 |
| SNRPN intron1 | chr15:22,752,948–22,753,011 | dG = −8.79,dH = −127.40,dS = −382.43,Tm = 60.0 |
| D15S12 | chr15:25,675,102–25,675,165 | dG = −1.24,dH = −38.40,dS = −114.99,Tm = 60.8 |
| OCA2 | chr15:26,017,987–26,018,049 | dG = −6.19,dH = −70.30,dS = −198.39,Tm = 81.2 |
| PWS26.04 Mb | chr15:26,048,967–26,041,021 | dG = −6.96,dH = −85.70,dS = −253.88,Tm = 64.4 |
| D15S1019 | chr15:27,355,543–27,355,605 | dG = −0.46,dH = −31.50,dS = −96.05,Tm = 54.8 |
| BP3distal | chr15:27,455,843–27,455,907 | dG = −0.43,dH = −22.40,dS = −67.99,Tm = 56.3 |
| D15S1048 | chr15:27,592,696–27,592,758 | dG = −4.37,dH = −119.50,dS = −356.27,Tm = 62.3 |
| D15S165 | chr15:28,985,442–28,985,500 | dG = −2.66,dH = −50.00,dS = −146.50,Tm = 68.2 |
| D15S1031 | chr15:29,809,165–29,809,225 | dG = −2.07,dH = −39.20,dS = −114.90,Tm = 68.0 |
| D15S1010 | chr15:30,784,010–30,784,069 | dG = −1.46,dH = −30.70,dS = −90.48,Tm = 66.1 |
| Beta actin | chr7:5,534,363–5,534,424 | dG = −8.82,dH = −112.70,dS = −334.93,Tm = 63.3 |
| D15S822 | chr15:24,873,458–24,873,521 | dG = −8.40,dH = −85.90,dS = −249.88,Tm = 70.6 |
| 15q14-5 | chr15:32,521,891–32,521,954 | dG = −2.10,dH = −70.60,dS = −220.86,Tm = 46.5 |
| 15q14-internal | chr15:32,568,078–32,568,140 | dG = −2.95,dH = −85.90,dS = −267.45,Tm = 48.0 |