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. Author manuscript; available in PMC: 2018 Jun 30.
Published in final edited form as: J Mol Biol. 2016 Nov 19;429(13):1924–1933. doi: 10.1016/j.jmb.2016.11.010

Figure 2. Structural basis for HIRA complex assembly and function.

Figure 2

(A) 1.7 Å crystal structure of S. cerevisiae Asf1 bound to X. laevis H3.1/H4 depicting the interaction of Asf1 with one H3/H4 dimer. (B) 2.7 Å crystal structure of the human HIRA/ASF1a complex showing association of the HIRA B-domain with the ASF1a core on the surface opposite to where ASF1a binds H3/H4. (C) 2.3 Å crystal structure of the UBN1/H3.3/H4/Asf1 complex revealing the molecular contacts between the UBN1 HRD and histone H3.3. (D) Structural model of the HIRA B-domain superimposed onto the UBN1/H3.3/H4/Asf1 complex depicting a hypothetical intermediate in the pathway of HIRA-mediated H3.3/H4 deposition.