Skip to main content
. 2017 May 19;4(5):054701. doi: 10.1063/1.4984052

FIG. 2.

FIG. 2.

dsDNA nuclease activity of XaCas2. (a) Cleavage of dsDNAs by XaCas2. Linearized pU19 plasmid was incubated with increasing amounts (5, 10, 20, and 40 μM) of XaCas2. (b) Metal dependence of the dsDNA nuclease activity of XaCas2. The dsDNA substrate was incubated with XaCas2 in the presence of divalent ions or EDTA. (c) pH dependence of the dsDNA nuclease activity of XaCas2. The substrate and XaCas2 were incubated in reaction buffer of different pHs. (d) Acetate ions in the proximity of the dsDNA substrate recognition loop. Three acetate ions incorporated during crystallization were identified near the β1–α1 loops of XaCas2 in the asymmetric unit. The acetate ions are shown in stick representations. The 2mFobs - DFcalc map is contoured at 1.0 σ for the acetate ions.