Table 3.
The relative concentrations of 16 plasma miRNAs chosen from TLDA determined by RT-qPCR assay for the SE, MH and MS protocols.
| miRNA | SE | MH | MS | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pre | 0 h | 1 h | 24 h | Pre | 0 h | 1 h | 24 h | Pre | 0 h | 1 h | 24 h | |
| miR-1 | 0.14 ± 0.02 | 0.17 ± 0.05 | 0.22 ± 0.04 | 0.12 ± 0.03 | 0.24 ± 0.05 | 0.19 ± 0.05 | 0.23 ± 0.05 | 0.33 ± 0.13 | 0.27 ± 0.06 | 0.24 ± 0.04 | 0.31 ± 0.06 | 0.29 ± 0.06 |
| miR-133a | 0.46 ± 0.09 | 0.46 ± 0.17 | 0.46 ± 0.11 | 0.43 ± 0.15 | 0.33 ± 0.04 | 0.17 ± 0.55* | 0.33 ± 0.06 | 0.28 ± 0.04 | 1.34 ± 0.30 | 0.90 ± 0.19* | 1.22 ± 0.27 | 1.17 ± 0.36 |
| miR-133b | 0.11 ± 0.02 | 0.12 ± 0.02 | 0.14 ± 0.03 | 0.11 ± 0.01 | 0.10 ± 0.01 | 0.08 ± 0.01 | 0.12 ± 0.04 | 0.14 ± 0.02† | 0.13 ± 0.02 | 0.11 ± 0.01 | 0.14 ± 0.02† | 0.11 ± 0.01 |
| miR-146a | 4.01 ± 0.21 | 3.65 ± 0.10 | 6.23 ± 2.01) | 4.17 ± 1.07 | 1.63 ± 0.38 | 1.15 ± 0.25 | 1.01 ± 0.21 | 1.26 ± 0.22 | 1.99 ± 0.31 | 2.33 ± 0.09 | 2.15 ± 0.10 | 1.79 ± 0.18 |
| miR-181a | 0.1 ± 0.02 | 0.08 ± 0.02 | 0.09 ± 0.01 | 0.07 ± 0.01 | 0.07 ± 0.02 | 0.07 ± 0.02 | 0.12 ± 0.03*,† | 0.06 ± 0.01‡ | 0.07 ± 0.01 | 0.06 ± 0.01 | 0.07 ± 0.01 | 0.05 ± 0.01 |
| miR-21 | 5.87 ± 0.67 | 4.35 ± 1.28 | 6.77 ± 0.99 | 4.89 ± 0.75 | 5.66 ± 0.79 | 3.93 ± 0.71* | 6.62 ± 1.00† | 5.17 ± 1.10 | 3.37 ± 0.63 | 3.03 ± 0.53 | 3.16 ± 0.52 | 2.63 ± 0.37 |
| miR-205 | 0.08 ± 0.01 | 0.06 ± 0.01 | 0.08 ± 0.01 | 0.08 ± 0.02 | 0.10 ± 0.01 | 0.12 ± 0.05 | 0.10 ± 0.01 | 0.10 ± 0.01 | 0.13 ± 0.02 | 0.13 ± 0.01 | 0.15 ± 0.02 | 0.14 ± 0.02 |
| miR-206 | 14.6 ± 2.32 | 13.1 ± 2.63 | 16.9 ± 3.23 | 13.1 ± 3.31 | 11.6 ± 1.60 | 8.9 ± 0.96 | 14.0 ± 1.34 | 10.7 ± 0.99† | 10.4 ± 1.61 | 8.2 ± 1.35 | 11.3 ± 1.90 | 7.3 ± 1.55 |
| miR-208b | 0.22 ± 0.04 | 0.13 ± 0.03* | 0.17 ± 0.03 | 0.12 ± 0.03* | 0.23 ± 0.06 | 0.17 ± 0.02 | 0.22 ± 0.07 | 0.24 ± 0.08 | 0.23 ± 0.03 | 0.17 ± 0.03 | 0.14 ± 0.02 | 0.16 ± 0.03 |
| miR-216a | ND | ND | ND | |||||||||
| miR-219 | 0.06 ± 0.01 | 0.05 ± 0.01 | 0.05 ± 0.01 | 0.04 ± 0.01 | 0.07 ± 0.01 | 0.06 ± 0.01 | 0.06 ± 0.01 | 0.07 ± 0.01 | 0.04 ± 0.01 | 0.05 (0.01) | 0.06 ± 0.01 | 0.06 ± 0.01 |
| miR-221 | 0.27 ± 0.02 | 0.31 ± 0.04 | 0.29 ± 0.03 | 0.24 ± 0.01 | 0.13 ± 0.01 | 0.16 ± 0.02 | 0.08 ± 0.69† | 0.15 ± 0.02‡ | 0.15 ± 0.07 | 0.12 ± 0.04 | 0.12 ± 0.05 | 0.12 ± 0.01 |
| miR-378 | 2.84 ± 0.30 | 2.48 ± 0.31 | 2.85 ± 0.49 | 2.54 ± 0.17 | 3.34 ± 0.41 | 4.08 ± 0.72 | 3.90 ± 0.56 | 3.73 ± 0.50 | 3.20 ± 1.03 | 3.05 ± 0.1 | 3.25 ± 0.45 | 2.98 ± 0.56 |
| miR-532 | 0.53 ± 0.08 | 0.61 ± 0.08 | 0.64 ± 0.08* | 0.69 ± 0.08* | 0.85 ± 0.57 | 0.77 ± 0.47 | 0.57 ± 0.28 | 0.86 ± 0.57 | 0.69 ± 0.05 | 0.58 ± 0.06 | 0.53 ± 0.08 | 0.50 ± 0.09 |
| miR-542 | 0.04 ± 0.01 | 0.03 ± 0.01 | 0.03 ± 0.01 | 0.03 ± 0.01 | 0.07 ± 0.01 | 0.06 ± 0.01 | 0.07 ± 0.01 | 0.06 ± 0.01 | 0.03 ± 0.01 | 0.03 ± 0.01 | 0.03 ± 0.01 | 0.03 ± 0.01 |
| miR-628 | 0.42 ± 0.03 | 0.39 ± 0.02 | 0.39 ± 0.04 | 0.34 ± 0.03 | 0.33 ± 0.06 | 0.34 ± 0.06 | 0.32 ± 0.05 | 0.29 ± 0.05 | 0.47 ± 0.02 | 0.42 ± 0.02 | 0.49 ± 0.04 | 0.41 ± 0.02 |
The relative concentrations of the circulating miRNAs were normalized to the Let-7d/g/i and presented as the mean ± SEM. miRNAs were obtained from plasma samples at baseline (Pre), immediately after exercise (0 h), after 1 h of recovery (1 h), after 24 h of recovery (24 h). *p < 0.05 compared to Pre; † p < 0.05 compared to 0 h; ‡ p < 0.05 compared to 1 h. ND, non-detected values. Values obtained from 15 subjects separately in each of the three groups.