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. 2017 May 19;7:2149. doi: 10.1038/s41598-017-02202-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EndophilinA triple knockouts show changes in BDNF-mediated signaling. (A) Western blots of the indicated proteins in EndophilinA1/3 double knockout (KWK refers to Knockout, Wild-type, Knockout, for EndophilinA1, A2, A3), and EndophilinA triple knockout (TKO) brain lysates compared to wild-type. (B) Quantitation of P-Akt, P-Erk, NFkB p65, GSK3 pS9, and pTrkB protein levels in Western blots normalized to actin; n = 3 independent experiments, significance determined by unpaired two-tailed Student’s t-test comparing each condition to control, error = SEM, **p < 0.01, ***p < 0.001. (C) Western blots of the indicated proteins in EndophilinA1/3 double knockout (KWK refers to Knockout, Wild-type, Knockout, for EndophilinA1, A2, A3), and EndophilinA triple knockout (TKO) hippocampal cultures compared to wild-type in control and BDNF-treated conditions (100 ng/ml BDNF for 30 minutes). (D) Quantitation of P-Akt, P-Erk, NFkB p65 and GSK3 pS9 protein levels in Western blots normalized to actin; n = 3 independent experiments, significance determined by unpaired two-tailed Student’s t-test comparing each condition to control, error = SEM, **p < 0.01, ***p < 0.001.