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. 2017 May 19;7:2155. doi: 10.1038/s41598-017-02204-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PP5 regulates the phosphorylation of FAK and ASK1 in wounded cells. Cell lysates from unwounded and wounded MLE-12 cells were collected after 24 hr, immunoprecipitated (IP) for FAK or ASK1, and then immunoblotted as indicated. Panel A Knockdown of PP5 using shRNA decreased p-Ser⁹⁶⁶-ASK1 in unwounded cells and showed no change in wounded cells. Panel B Knockdown of PP5 using shRNA caused a significant increase in p-Ser-FAK (recognized by the 1C8 antibody) in wounded cells. Right panels show the densitometry analysis normalized to unwounded controls. Asterisks (*) indicate a significant difference compared to control, (n = 4), p < 0.05. N.S. – no significant difference. Each set of bands shown was cropped from the same blot, and the full-length blots were provided in supplemental data. Each protein indicated was immunoblotted separately from independent gels.