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. 2017 May 19;7:2172. doi: 10.1038/s41598-017-02057-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Musashi-1 inhibits 5-FU-induced apoptosis in HCT-116 cells. (A) Upper panel: FLAG, FLAGMusashi-1, ΔR1FLAGMusashi-1, and ΔCFLAGMusashi-1 HCT-116 stable clones were seeded on 22 mm × 22 mm coverslips and treated with 5-FU (400 μM) for 24 h. Cells were fixed in 4% paraformaldehyde and subjected to the TUNEL assay as described in the Methods. Lower panel: Statistical results of percentages of TUNEL positive cells. Error bars indicate the mean ± SD from three independent experiments. ^#p < 0.05. (B) Upper panel: FLAG, FLAGMusashi-1, ΔR1FLAGMusashi-1, and ΔCFLAGMusashi-1 HCT-116 stable clones were treated with or without 5-FU (400 μM) for 24 h. Total cellular proteins were isolated and subjected to immunoblotting with antibodies specific to PARP. Increased cleaved PARP (c-PARP) signals were observed in ΔCFLAGMusashi-1 HCT-116 stable clones. Tubulin indicates Tubulin protein as an internal control. Lower panel: Statistical results of percentages of relative intensity of c-PARP. Error bars indicate the mean ± SD from three independent experiments. ^#p < 0.05.