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. 2017 May 19;7:2172. doi: 10.1038/s41598-017-02057-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased Musashi-1 expression in CRC cells. Paired clinical samples (one stage IIA and two stage IIB samples) of normal and CRC tissues were subjected to Musashi-1 immunohistochemical staining as described in the Methods. Relative Musashi-1 expression levels were detected by a Musashi-1-specific antibody and fluorescence dye-conjugated secondary antibodies.