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. 2017 May 19;7:2141. doi: 10.1038/s41598-017-01490-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Construction and characterization of the P. aeruginosa bioD::Gm strain. (a) Recombination events required to generate the mutant strain by sacB counterselection gene replacement. (b) Characterization of the mutation by biotin complementation. Strain XC.059 was streaked on M9 defined medium plates containing 40 nM biotin (left plate) or lacking biotin (right plate) Abbreviations: kan, kanamycin-resistant gene; Gm^R, Gm resistant marker; ori, pMB1 origin of replication; oriT, origin of transfer; sacB, levansucrase-encoding gene.