Table 3.
Quantification of UCH-L1 protein expression in tumors and their paired tissues on western blot.
| proteins | UCHL-1 | CDK4 | CaSR | β-actin | *Ratio of UCH-L1/β-actin | IHC result |
|---|---|---|---|---|---|---|
| intensity | ||||||
| sample # | ||||||
| #4 | 1450 | / | / | 1224 | 1.18 | + |
| # 289 | 492 | / | / | 459 | 1.07 | + |
| #44 | 660 | 155 | 1645 | 835 | 0.79 | + |
| #290 | 713 | 290 | 15 | 880 | 0.81 | + |
| #67 | 171 | 656 | 0 | 1335 | 0.13 | + |
| #5 | 434 | 754 | 0 | 1267 | 0.34 | + |
| #5 N | 32 | 12 | 0 | 777 | 0.04 | − |
| Nor #1 | 0 | 206 | 0 | 631 | 0 | − |
| Nor #2 | 0 | 116 | 0 | 458 | 0 | − |
| Nor #3 | 0 | 0 | 0 | 365 | 0 | − |
*The ratio of UCH-L1/β-actin in 6 tumor specimens (#4, #289, #44, #290, #67 and #5) was significantly higher than that in paired tissue (#5 N) or other normal pancreatic tissues (Nor 1, 2, 3), median 0.8 (0.13–1.18) vs. 0 (0–0.04), p = 0.0095, Mann-Whitney U test. The results from western blot are comparable with that from immunohistochemical (IHC) staining.