Figure 5.

(a) Total number of peptide uptake values successfully calculated from three, four-point, HDX time-course experiments when acquired in MS (Cambridge blue) and IM-MS (grey) modes. Peptide were removed from initial peptide maps if ion interferences were present or the identity of the ion could not be confirmed based on RT, m/z, and arrival time (if available). (b) Example of the IMS separating interfering ions of a different charge state from the peptide ion of interest, allowing the calculation of accurate uptake values. MS data (blue) and IM-MS data (black) shown have been extracted within DynamX using identical settings. The area highlighted in grey denotes the expected m/z region for the undeuterated peptide ion