Fig. 5.

Inhibition of human platelet adhesion to collagen by MHCSZ-123 under shear stress. Heparinized whole blood was perfused over the collagen substrate at a constant high (1200 s⁻¹) or low (300 s⁻¹) shear rate at 37 °C for 5 min. The fluorescence microscope images of platelet adhesion at 0, 1, 3, and 5 min of perfusion under high or low shear stress are presented (10 × 20). a MHCSZ-123 inhibited VWF binding to collagen under high shear stress, similar to the parent mAb SZ-123. b Neither MHCSZ-123 nor SZ-123 inhibited VWF binding to collagen under low shear stress. The concentration of antibodies was 10 μg/ml. The non-inhibitory antibody SZ-34 (10 μg/ml) was used as a control