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. 2017 May 19;10:132. doi: 10.1186/s13068-017-0819-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Production of rhTrEG I and rhTrEG II in Y. lipolytica a enzyme production on YTD versus time, b SDS-PAGE analysis of the culture supernatant of Y. lipolytica transformants compared with the control, and c Western blot analysis; lanes 1 and 3, culture supernatant of transformant ylEGI and ylEGII, respectively; lanes 2 and 4, culture supernatant of transformant ylEGI and ylEGII treated by endo-H, respectively