Table 2.

Cycle thresholds for Plasmodium 18S rRNA, PF3D7_0630000 and pfs25 in gametocyte dilution series

Gametocytes/mL	Plasmodium 18S rRNA	PF3D7_0630000	pfs25
3 × 107	5.40	15.70	10.53
3 × 106	9.36	19.70	14.48
	8.85	19.38	13.94
3 × 105	12.16	23.13	17.91
	12.34	22.98	17.42
3 × 104	15.12	26.47	20.70
	15.11	26.50	20.65
3 × 103	18.14	29.43	23.60
	18.63	29.60	23.99
3 × 102	21.85	33.86	27.74
	21.82	33.65	27.92
3 × 101	24.06	37.89	30.35
	ND	ND	ND
3 × 100	ND	ND	ND
	ND	ND	ND

Plasmodium 18S rRNA RT-PCR LOD = 20 parasites/mL

ND, not detected; All in duplicate except 3 × 10⁷ gametocyte/mL sample