Table 1.
Primers
| Primers to create DARC-eGFP fusion protein | ||
|---|---|---|
| DARC Fwd | 5′- GCC ACC ATG GGG AAC TGT CT | |
| DARC Rvs | 5′-GGA CTT GCC TGC AAG GGC AT | |
| BamH1-DARC Fwd | 5′- GGA TCC GCC ACC GCC ACC ATG GGG AAC TGT CTG TAT C | |
| DARC-linker1 Rvs | 5′- CCC TTG CTC ACC ATC TCG AGG GAC TTG CCT GCA AGG GCA TC | |
| DARC-linker3 Rvs | 5′- CGC CGC GCT GCC GCC GCC GCC GGA CTT GCC TGC AAG GGC ATC | |
| GFP-linker1 Fwd | 5′- TTG CAG GCA AGT CCC TCG AGA TGG TGA GCA AGG GCG AGG AG | |
| GFP-linker3 Fwd | 5′- GGC GGC GGC AGC GCG GCG GCG ATG GTG AGC AAG GGC GAG GAG | |
| GFP-ECORI Rvs | 5′- GAA TTC TTA CTT GTA CAG CTC GTC CAT GCC G | |
| Primers for RT-qPCR | ||
| GAPDH Fwd | CCAATGTGTCCGTCGTGGATCT | |
| GAPDH Rvs | GTTGAAGTCGCAGGAGACAACC | |
| DARC Fwd | TCCGGTGGAAAACCTTTCACTA | |
| DARC Rvs | GCTGGTGTCAGGCTGTAGTC | |
| Selp Fwd | TGTTTGGCTTCTGGGATCTGGACA | From Kokkinaki et al. [69] |
| Selp Rvs | AGGCAGCAATTGGGTGCATACAG | |
| Madcam1 Fwd | GACCCATAGAAAGGAGATTCCAGTA | |
| Madcam1 Rvs | TGAGCCCAGTGGAGACTG | |
| Sele Fwd | TGAACTGAAGGGATCAAGAAGACT | From McEver et al. [70] |
| Sele Rvs | GCCGAGGGACATCATCACAT | |
| Chst4 Fwd | TGCCCCACCTCCAAACAT | From Ruddle et al. [71] |
| Chst4 Rvs | GACCAACGCCACGCCTGAGA | |
| Fut7 Fwd | GGACCTCCTCGGGCCACCTACG | From Ruddle et al. [71] |
| Fut7 Rvs | CGCCAAGCAAAGAAGCCACGATAA | |