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Buffer recipes
| Buffer name | Recipe |
|---|---|
| IHC blocking buffer | PBS with 0.5% BSA (Calbiochem) and 0.3% Triton X-100 (Sigma) |
| IHC washing buffer | PBS with 0.2% BSA (Calbiochem) and 0.1% Triton X-100 (Sigma) |
| Digestion buffer | HBSS (Corning) with 2% FBS (Gemini), 10 mM HEPES (Corning) and 2 mM CaCl2 (Sigma) |
| FACS buffer | PBS (Lonza) with 5% FCS (Invitrogen-Gibco) and 5 mM EDTA (Boston BioProducts) |
