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. 2017 May 19;17:352. doi: 10.1186/s12885-017-3340-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Analysis of Notch pathway activation in neuroblastoma cells with different MYCN gene amplification. SH-SY5Y and IMR-32 cells were chosen as the two cell lines with the lowest and the highest N-Myc mRNA expression. a Confocal analysis of NICD expression levels in SH-SY5Y and IMR-32 cells. β III tubulin antibody (green) was used as neuron marker; NICD antibody (red) showed a typical nuclear localization, indicative of Notch activation. b Western blot analysis of NICD protein expression in SH-SY5Y and IMR-32 cells. c Densitometric analysis of NICD expression levels in SH-SY5Y and IMR-32 cells using Quantity One analysis software. *p < 0.05 vs SH-SY5Y cell line