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. 2017 May 19;17:352. doi: 10.1186/s12885-017-3340-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Analysis of miRNA-34b transfection in IMR-32 neuroblastoma cells line. a Confocal analysis of miRNA-34b transfection in IMR-32 neuroblastoma cells after 3 days of transfection. β III tubulin antibody was used as neuron marker. b Morphometric analysis of cell differentiation induced by miRNAs transfection. IMR-32 neuroblastoma cells underwent 3 days of transfection and then neuronal differentiation was evaluated by measuring neurite length. The percentage of differentiated cells was calculated, considered as cells with neurites ≥50 μM in length in IMR-32 cells. *p < 0.05 vs control