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. 2017 May 19;16:41. doi: 10.1186/s12941-017-0215-z

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Filastatin inhibits attachment of C. albicans to bioactive glass. C. albicans was incubated in varying concentrations of Filastatin in 24 well plates containing bioactive glass coupons. a Direct cell counts were obtained by propidium iodide and Syto9^® staining. Black bars represent the number of wild type cells (control) or non-adherent cells (edt1 ^−/−) that attach to untreated bioactive glass. Grey and white bars represent wild-type cells treated with varying concentrations of Filastatin. Standard error bars displayed, p < 0.0001. b Crystal violet staining to determine adhered cells to the surface of bioactive glass coupons