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. 2017 Feb 16;8(17):27676–27692. doi: 10.18632/oncotarget.15398

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Copyright: © 2017 Ma et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. The mice began to receive FLX treatment at 64-68 weeks (16-17 months) of age, and the treatment was continued until they reached 69-73 weeks (17.25-18.25 months) of age, with the FLX administration lasting for 5 weeks. The Morris water maze was conducted when mice were at 68-72 weeks (17-18 months) of age, after being administered fluoxetine for 4 weeks. B. Six mice each were randomly chosen from the WT group and the APP/PS1 group, and five mice were randomly chosen from the APP/PS1+FLX group for the subsequent stereological investigation and immunohistochemistry. In each group, those mice not used for the stereological investigation and immunohistochemistry were deeply anesthetized and then killed. Then, the hippocampus was obtained from each brain and homogenized for western blotting analysis.