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. 2017 Feb 6;8(17):27772–27785. doi: 10.18632/oncotarget.15115

Figure 5. JNK activation was involved in B392-caused cell apoptosis.

Figure 5

(A) HL60 cells were treated with vehicle (0.1% DMSO) or B392 (0.1 μM) for time course. The cells were harvested for detection of indicated proteins by western blot analysis. (B) HL60 cells were exposed to B392 (0.1 μM) in the absence or presence of SP600125 (10, 20 μM) for 18, 24, 36 and 48 hours. Cell cycle distribution was analyzed by flow cytometry as well as determining (C) the percentage of subG1 cell number. (***P < 0.001) (D) The expression of indicated proteins was determined after treatment of HL60 with 0.1 μM B392 in the absence or presence of SP600125 (10, 20 μM) pretreatment for 30 min. SP: SP600126, p-JNK inhibitor.